CCL-35 Pt K1 （NBL-3） 袋鼠腎細胞，ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻i優(yōu)培養(yǎng)條件
CCL-35 Pt K1 （NBL-3） 袋鼠腎細胞的詳細介紹
CCL-35
| ATCC® Number: | CCL-35™ | Price: | $438.00 |
| Designations: | Pt K1 （NBL-3） [PTK1] |
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| | Depositors: | KH Walen |
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| | Biosafety Level: | 1 |
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| | Shipped: | frozen |
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| | Medium & Serum: | See Propagation |
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| | Growth Properties: | adherent |
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| | Organism: | Potorous tridactylis （potoroo） |
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| | Morphology: | epithelial
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| | Source: | Organ: kidney
Disease: normal |
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| | Cellular Products: | keratin |
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| | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
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| | Virus Resistance: | poliovirus 2 |
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| | Age: | adult |
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| | Gender: | female |
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| | Comments: | The cells are positive for keratin by immunoperoxidase staining. |
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| | Propagation: | ATCC complete growth medium: Minimum essential medium （Eagle） in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; newborn bovine calf serum, 10% |
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| | Subculturing: | Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per weekRinse cell sheet twice with 0.25% trypsin, 0.03% EDTA （or Alsever's Trypsin Versene） solution, remove the trypsin solution, and allow the culture to stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks. |
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| | References: | 26188: . . Nature 194: 406, 1962. 26189: . . Cytogenetics 3: 19, 1964. |
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