CCL-33 PK-15 豬腎細(xì)胞系,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件
CCL-33 PK-15 豬腎細(xì)胞系 的詳細(xì)介紹
| ATCC® Number: | CCL-33™ | Price: | $355.00 |
| Designations: | PK（15） |
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| | Depositors: | Cutter Laboratories, Inc. |
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| | Biosafety Level: | 1 |
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| | Shipped: | frozen |
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| | Medium & Serum: | See Propagation |
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| | Growth Properties: | adherent |
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| | Organism: | Sus scrofa （pig） |
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| | Morphology: | epithelial |
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| | Source: | Organ: kidney
Disease: normal |
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| | Cellular Products: | plasminogen activator; keratin |
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| | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
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| | Applications: | transfection host （Roche FuGENE® Transfection Reagents） |
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| | Virus Resistance: | poliovirus 2 |
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| | Age: | adult |
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| | Comments: | The presence of a porcine papovavirus in PK（15） cells has been reported in cells obtained from multiple sources including the ATCC . [53398] [53399] The Foreign Animal Disease Diagnostic Laboratory of the US Department of Agriculture has determined that ATCC CCL-33 is not infected with Hog cholera virus or African swine fever virus, and uses this line to screen for those viruses. [105588] The cell line harbors an endogenous C-type retrovirus. [26185] [53399] [56104] The cells are positive for porcine circovirus （PCV） antigens. The cells are positive for keratin by immunoperoxidase staining. |
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| | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C |
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| | Subculturing: | Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per weekRinse the cell sheet 2 times with fresh 0.25% trypsin, 0.03% EDTA solution, remove trypsin and allow the culture to stand at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. |
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| | Preservation: | culture medium 95%; DMSO, 5% |
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| | Related Products: | Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2003 recommended serum:ATCC 30-2020 |
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| | References: | 22988: Dulac GC, Afshar A. Porcine circovirus antigens in PK-15 cell line （ATCC CCL-33） and evidence of antibodies to circovirus in Canadian pigs. Can. J. Vet. Res. 53: 431-433, 1989. PubMed: 2686830 26184: Pirtle EC, Woods LK. Cytogenetic alterations in swine kidney cells persistently infected with hog cholera virus and propagated with and without antiserum in the medium. Am. J. Vet. Res. 29: 153-164, 1968. PubMed: 4965860 26185: Armstrong JA, et al. C-type virus particles in pig kidney cell lines. J. Gen. Virol. 10: 195-198, 1971. PubMed: 4324256 53398: Newman JT, Smith KO. Characteristics of a swine papovavirus. Infect. Immun. 5: 961-967, 1972. PubMed: 4344097 53399: Tumilowicz JJ, et al. Concurrent replication of a papovavirus and a C-type virus in the CCL 33 porcine cell line. In Vitro 15: 922-928, 1979. PubMed: 232060 56104: Todaro GJ, et al. Characterization of a type C virus released from the porcine cell line PK（15）. Virology 58: 65-74, 1974. PubMed: 4132403 105588: James A House, personal communication |
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